Figure 5

Wnt3a, Wnt7a, and Wnt7b activate the canonical pathway in hippocampal cultures. (A-D) Representative images of 'active' β-catenin stabilization by endogenous Wnts (A), addition of recombinant Wnt3a (B; 150 ng/mL), and addition of recombinant Wnt7a (C; 200 ng/mL), or Wnt7b conditioned media (D). Scale bar = 6 μm. (E, F) Quantification of 'active' β-catenin immunofluorescence intensity by Wnts in the nucleus (E) and soma (F). Each condition was normalized to the control condition. (n = 428 cells; *p < 0.001, t-test for each control and experimental group pairing, error bars represent SEM). MAP2, Microtubule associated protein 2.