Subcellular Fzd3 expression. (A) An image of a green fluorescent protein (GFP) transfected neuron in a low density area stained for GFP (dark blue) and Fzd3 (green). Areas of overlap appear as light blue. (B) Sections on this neuron show that Fzd3 is expressed in both axons (B; left) and dendrites (B; right). (C) Staining of a GFP transfected neuron with VGLUT1 (red), Fzd3 (green), and GFP (dark blue). Some excitatory presynaptic puncta colocalize with Fzd3 (arrows) and some do not (asterisks). Scale bar = 2 μm.