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Figure 1 | Neural Development

Figure 1

From: Genetic lineage tracing defines distinct neurogenic and gliogenic stages of ventral telencephalic radial glial development

Figure 1

Glast bacterial artificial chromosome (BAC) transgenic mice recapitulate the spatiotemporal pattern of endogenous GLAST (glial high affinity glutamate transporter) expression. (e-v") In situ hybridization (ISH) (a-d) and immunofluorescent staining (e-v) of Glast::Cre (a-i) and Glast::eGFP (j-v) BAC transgenic mice. Cre mRNA in the ganglionic eminences (a), thalamus (b), spinal cord (c), and cerebellum/hindbrain (d) was completely restricted to the ventricular zone, with no neuronal expression detected at any age examined. The Glast::Cre transgene also recapitulated endogenous GLAST expression gradients (compare (c) and (r)). Postnatally, Cre immunoreactivity (green) (e-i) was restricted to astroglia. Shown are cortical GLAST+ astrocytes (f), striatal BLBP+ astrocytes (g), and cerebellar Bergmann and astroglia (h,i); no colocalization with CALB1+ Purkinje cells was observed (i). Double immunofluorescent staining of Glast::eGFP embryos for GLAST (red) and enhanced green fluorescent protein (eGFP; green) showed that the BAC transgene drives expression in essentially all GLAST+ radial glia (j-q); the notable exception to this was the spinal cord floorplate where no transgene expression was detectable (r-t). Postnatal expression was similarly restricted to astroglia (u.v). Scale bars: 200 μm (c,d,e,h,r,s); 100 μm (b); 75 μm (a,j-l); 50 μm (f,n-p,t); 40 μm (m); 35 μm (u); 25 μm (g,i,q,v). E, embryonic day.

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