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Table 2 Motor axon phenotypes in Rptp double and triple mutant embryos

From: Redundancy and compensation in axon guidance: genetic analysis of the Drosophila Ptp10D/Ptp4E receptor tyrosine phosphatase subfamily

  Phenotype (%)*
Genotype       
ISN n t T SB FB  
   Ptp4E1Ptp10D1 155 6 3 3 0  
   Ptp4E1; Ptp52F18.3 126 12 2 8 2  
   Ptp10D1; Ptp52F18.3 207 67 40 23 4  
   Ptp4E1Ptp10D1; Ptp52F18.3 203 74 11 60 3  
   Dlar5.5/Dlar13.2 256§ 41 22 19 0  
   Ptp4E1; Dlar5.5/Dlar13.2 91 51 30 21 0  
   Ptp4E1Ptp10D1; Dlar5.5/Dlar13.2 133 60 32 26 2  
ISNb n t B S C  
   Ptp4E1Ptp10D1 119 2 0 2 0  
   Ptp4E1; Ptp69D1/Df(3L)8ex25 106 11 8 3 0  
   Ptp10D1; Ptp69D1/Df(3L)8ex25 101 94 4 90 0  
   Ptp4E1Ptp10D1; Ptp69D1/Df(3L)8ex25 108 93 1 36 56  
SNa n t M S A -
   Ptp4E1Ptp10D1 139 5 3 1 1 0
   Ptp4E1; Ptp69D1/Df(3L)8ex25 122 7 5 1 1 0
   Ptp10D1; Ptp69D1/Df(3L)8ex25 85 26 13 10 2 1
   Ptp4E1Ptp10D1; Ptp69D1/Df(3L)8ex25 106 41 18 14 0 9¥
  1. n, number of hemisegments (A2–A7) scored; t, all affected branches. For ISN phenotype categories: T, ISN branches that end at the normal terminal arbor position but are thin or bifurcated; SB, ISNs that terminate at the second branchpoint; FB, ISNs that terminate at the first branchpoint. For ISNb phenotype categories: B, ISNb branches that bypass the ventrolateral muscle field (muscles 6, 7, 12, 13) and grow adjacent to ISN or fail to exit the ISN pathway and are fused to ISN; S, ISNbs that stall within the VLM field or fail to enter the VLM field; C, ISNbs that stall with a clump. For SNa phenotype categories: M, either posterior or anterior branch is missing; S, SNa branches stall near the bifurcation point; A, additional branches;-, the whole branch is very thin or absent. *The numbers listed are percent of hemisegments that display a phenotype. The results for Ptp10D1; Ptp52F18.3 were published in [5]. For ISN motor axons, the differences between the triple mutant and each of the double mutants (Ptp4E Ptp52F and Ptp10D Ptp52F) are statistically significant (p < 0.0001, Chi-square test). §The results for Dlar5.5/Dlar13.2 were published in [19]. While approximately 90% of ISNbs end at the base of m13 in both genotypes, the phenotype is different in the triple mutant: 56% of stalled ISNb axons in triple mutants form a clump, whereas in the double mutant they simply resemble earlier (stage 16) axons with growth-cone like morphologies. These embryos were scored with genotypes blinded by another observer to make sure we could distinguish the phenotypes. The difference between the triple and the double mutants is highly statistically significant (p < 0.0001, Chi-square test). ¥Difference between the triple and the double mutant (Ptp10D Ptp69D) is statistically significant (p < 0.0001, Chi-square test).