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Figure 7 | Neural Development

Figure 7

From: Crucial roles of Robo proteins in midline crossing of cerebellofugal axons and lack of their up-regulation after midline crossing

Figure 7

Failure of cerebellofugal (CF) axons crossing the midline in Rig-1 knockout mice. (A-D) Transverse sections including the circumferential CF axon pathway were prepared from embryonic day 13 wild-type (A,C), Rig-1 heterozygous (B) and homozygous (D) mice and stained with anti-Rig-1 (A) or anti-TAG-1 (B-D) antibodies. Both Rig-1 (A) and TAG-1 (B) were expressed by CF axons (arrows) running from the cerebellar plate (CP) toward the floor plate (FP) in wild-type mice. TAG-1 positive axons were not observed around the FP in Rig-1 homozygotes (D), indicating that commissural axons, including CF axons (arrow), fail to cross the FP. Arrowhead in D indicates TAG-1 positive axons coursing longitudinally. No stained axons can be seen near the midline (asterisk). Dorsal side is to the top in all panels. Scale bar in (D) is 200 μm and applies to all panels.

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