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Figure 2 | Neural Development

Figure 2

From: Crucial roles of Robo proteins in midline crossing of cerebellofugal axons and lack of their up-regulation after midline crossing

Figure 2

(A,B) In situ hybridization for Robo1 (A) and Robo2 (B) in coronal sections of the cerebellar plate (CP). Both Robo1 and Robo2 mRNAs are expressed in the region where deep cerebellar nucleus neurons should be located (arrows). d is dorsal and v is ventral. (C) Immunostaining, in an adjacent section, for Meis 2, a transcription factor known to be expressed in developing deep cerebellar neurons. (D) Coronal section of an E13 rostral hindbrain that was in utero electroporated at embryonic day 11 to introduce egfp plasmid into the vz and upper rhombic lip of the cerebellar plate. Axons can be seen to emanate from cell bodies located in the nuclear transitory zone and project ventrally to cross ventral midline. (E) egfp+ axons in the ventral midline region. (F) Robo1 immunostaining of the same section as in (E) shows numerous Robo1+ circumferential axons at the midline region. (G-I) Higher power and merged views of boxed areas in (E,F). At least a subset of egfp+ axons (arrowheads in G) expressed Robo1 (arrowheads in H; see also merged image in I). vz, ventricular zone. Arrows in (D-F) indicate ventral midline. Scale bar = 200 μm in (C), which applies to (A-C); 300 μm in (D); 300 μm in (E,F); 75 μm in (G-I).

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