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Figure 1 | Neural Development

Figure 1

From: Crucial roles of Robo proteins in midline crossing of cerebellofugal axons and lack of their up-regulation after midline crossing

Figure 1

Expression patterns of Robo1 and Robo2 proteins in the rostral hindbrain. (A-F) Embryonic rat hindbrain was flat, whole-mounted and immunostained for Robo1 (A,C,D,E) and Robo2 (B,F). The whole-mount preparations were placed with the ventricular side down. (A-D) are from embryonic day (E)13 and (E-F) are from E16 rat embryos. Both Robo1 and Robo2 proteins are largely expressed by longitudinally growing axons. (A) At E13, when cerebellofugal axons have just left the cerebellar plate (CP) [5], strong expression of Robo1 is seen in longitudinal axons. (C,D) Higher magnifications of areas shown by rectangles in (A) in the CP (C) and the midline floor plate (FP) (D), respectively. A small number of midline-crossing axons can be seen in the FP (arrow in D). (B) Immunostaining for Robo2. Robo2 immunopositive fibres also run longitudinally but in a region far from the FP. At E16, both Robo1 and Robo2 immunopositive fibres run more or less longitudinally in overlapping regions (E,F). Scale bars = 200 μm; the bar in (B) also applies to (A); that in (D) also applies to (C); and that in (F) also applies to (E).

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