Figure 8

In situ hybridisation for neurofilament (blue) combined with tyrosine hydroxylase (TH)-immunocytochemistry (red) on sections of sympathetic ganglia at the upper thoracic level (A,B) and of the adrenal gland (C) of embryonic day (E)8 chick embryos: (A,C) control embryo; (B) embryo that was transplanted with bone morphogenetic protein-4 (BMP-4)-expressing fibroblasts at E2. Note that the majority of cells in the sympathetic ganglia of both control and BMP-4-overexpressing embryos is neurofilament-positive, while in the adrenal gland most cells are TH-positive, but neurofilament-negative. (D) The percentage of TH-positive neurofilament-negative cells was not altered in BMP-4-overexpressing embryos. Bars: 100 μm.