Figure 6

Quantification of tyrosine hydroxylase (TH)-immunoreactive cells in adrenal explant cultures after 0, 1, 3, and 5 days in vitro with or without noggin-treatment. Numbers of TH-positive cells in explants treated with noggin failed to increase, in contrast to those in control cultures. Counts of TH-immunoreactive cells were performed in every sixth section of the adrenal explant cultures. Data are presented as means ± standard error of the mean. At least seven explants for each group were analyzed.