Figure 6

Modulation of Fasciclin II (FasII) levels by Fak56. (A-C) Expression of FasII (green) at neuromuscular junction (NMJ) 6/7 in elav>LacZ (A1), elav>Fak56RNAi (B1), and elav>Fak56RNAi;rl^{EMS 698/+}(C1). Co-stained horseradish peroxidase (HRP) is in magenta. (A2-C2) Only FasII staining is shown. Images in (A-C) come from a single section of the Z-stack confocal image. (D) Quantification of FasII levels relative to HRP immunoreactivity shown in (A1-C1). Note that elav>Fak56RNAi had a 33.5% reduction compared to elav>LacZ, which was restored significantly by removing one copy of rl in elav>Fak56RNAi;rl^{EMS 698/+}. Asterisks indicate significant difference by Student's t test (p < 0.05) and error bars represent the standard error of the mean (SEM).