Figure 5

Suppression of ERK activity by Fak56 during neuromuscular junction (NMJ) growth. (A, B) Immunostaining of elav>LacZ (A1) and elav>Fak56RNAi (B1) for diphospho-ERK (dpERK; green) and horseradish peroxidase (HRP; magenta). A single section of image is shown. Punctate expression of dpERK was observed in presynaptic boutons and enhanced in the enlarged NMJ 4 in elav>Fak56RNAi. (A2, B2) Only dpERK expression is shown. Scale bars are 10 μm. (C) Quantification of relative immunoreactivities of dpERK to HRP within the presynaptic zone. Note that elav>Fak56RNAi and elav>Fak56^{Y 430F}had 3.3- and 3.1-fold increases compared to elav>LacZ. (D, E) Images of NMJ 6/7 are shown as described in Figure 1. (E) Depletion of Fak56 activity in elav>Fak56RNAi results in NMJ overgrowth, which can be suppressed by the rl^{EMS 698}allele (F). (F) Quantification of NMJ 6/7 phenotypes for the control elav>LacZ, elav>Fak56RNAi, elav>Fak56RNAi;rl^{EMS 698/+}and rl^{EMS 698/+}. Note that the rl^{EMS 698}allele suppressed NMJ phenotypes in elav>Fak56RNAi. Asterisks indicate significant difference by Student's t test (p < 0.05) and error bars represent the standard error of the mean (SEM).