Figure 5
From: Loss of transforming growth factor-beta 2 leads to impairment of central synapse function
Immunohistochemical staining, synaptic number and size of neurons in the pre-Bötzinger-complex (preBötC) area. (a) Immunohistochemical staining of pre- and postsynaptic marker proteins of synapses in the preBötC area in wild-type (WT) and knock-out (KO) brain sections. Scale bar, 20 μm. (b) Quantification of positive punctae in WT and KO animals in the preBötC area. (c) Size of positive punctae in WT and KO animals in the preBötC area. (d) Nissl-stain to identify sections containing the nucleus ambiguus (arrowhead) and staining with anti-neurofilament (NF)150-antibody in WT and KO brain sections. Insets show higher magnifications of the nucleus ambiguus that were used to identify the region of the preBötC. Scale bar, 500 μm. SEM, standard error of the mean. **p < 0.01