Table 2 Antibodies used in this study
From: A synaptic nidogen: Developmental regulation and role of nidogen-2 at the neuromuscular junction
Antigen | Isotype | Source | Dilution | Reference |
|---|---|---|---|---|
Nidogen-1 | Rabbit IgG | Abcam, Inc. | 1:2000 | Figure 1 |
Nidogen-2 | Rabbit IgG | Abcam, Inc. | 1:2000 | Figure 1 |
znp-1 (Synaptotagmin 2) | Mouse IgG2a | Zebrafish International Resource Center | 1:200 | |
Collagen α3(IV) | Rat IgG | Gift of Y Sado (Shigei Medical Research Institute, Okayuma) | 1:100 | [78] |
Collagen α4(IV) | Rat IgG | Gift of Y Sado | 1:100 | [78] |
Collagen α5(IV) | Rabbit IgG | Generated in our lab | 1: 2000 | [11] |
Collagen α6(IV) | Rat IgG | Gift of Y Sado | 1:25 | [78] |
Laminin β2 | Rabbit IgG | Gift of T Sasaki and R Timpl (Max Plank Institute, Munich) | 1:1000 | [79] |
Laminin α4 | Rabbit IgG | Kind gift of T Sasaki and R Timpl | 1:1000 | [80] |
Laminin α5 | Rabbit IgG | Generated in our lab | 1:2000 | [81] |
Agrin | Rabbit IgG | Gift of Z Hall (UCSF) | 1:300 | [82] |