Figure 8

Nidogen-2 phenotypes vary among muscles. (A-C) Neuromuscular junction (NMJ) morphology was differentially affected in different muscles. Like diaphragm (Dia) (A), most NMJs in soleus (Sol) muscles were fragmented or immature (B), whereas NMJs in tibialis anterior (TA) and extensor digitorum longus (EDL) appeared less affected (C, D). NMJs were labeled with only α-bungarotoxin. (E) Quantification of NMJ morphology in several different mutant and control muscles. Y-axis represents the percentage of NMJs appearing either pretzel-like (that is, normal), fragmented (blue, as in Figure 6B, D, E), or immature (red, as in Figure 6C). Control tibialis anterior, n = 92 NMJs from 3 animals. Control diaphragm, n = 100 NMJs from 3 animals. Nid2^-/- EDL, n = 105, from 3 animals. Nid2^-/- tibialis anterior, n = 91, from 3 animals. Nid2^-/- soleus, n = 129, from 3 animals. Nid2^-/- diaphragm; n = 384, from 3 animals. Scale bar in (D) is 25 μm for (A-D).