Figure 5
From: A synaptic nidogen: Developmental regulation and role of nidogen-2 at the neuromuscular junction
Aberrant neuromuscular junction (NMJ) morphology in the absence of nidogen-2. NMJs from diaphragms of P56 nid2-/- mutants and aged-matched controls. Pre- and postsynaptic elements are labeled with anti-synaptotagmin 2 (syt 2) and α-bungarotoxin (BTX). (A) In controls, NMJs appeared pretzel-like. (B-E) In mutants, NMJs were frequently fragmented into small clusters (B, D, E) or appeared plaque-like (C). Despite topological abnormalities, mutant pre- and postsynaptic elements remain precisely aligned (B"). Scale bar in (A) is 10 μm for all parts.