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Figure 5 | Neural Development

Figure 5

From: A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation

Figure 5

SAD-1 kinase establishes synaptic organization. (A) Developmental exposures. SAD-1as animals were exposed to 1NA-PP1 throughout establishment (Ai-ii) or partially during establishment (Aiii) (filled bars). Observations were made at an adult stage (Ai) or immediately following the exposures (Aii-iii) (solid lines). (B) Synaptic organization phenotypes following the exposures. The two-day establishment exposure to 1NA-PP1 had no effect on the SAD-1as-induced rescue observed at the adult stage (Bi). However, when SAD-1as animals were observed immediately after treatment, the rescuing effects of SAD-1as were abolished (Bii-iii). Quantification and density estimates of the punctum widths show that in the adult stage, DMSO (black) and 1NA-PP1 (red) treatments appeared identical and rescued compared to sad-1 mutants (Biv). In contrast, immediately after 1NA-PP1 treatment, obvious changes in the distribution of the punctum widths were observed (Bv-vi). All density graphs show widths on the x-axis and density on the y-axis. Scale bar, 5 μm.

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