Figure 5
From: Expressing exogenous functional odorant receptors in cultured olfactory sensory neurons
Over-expressed mouse I7 odorant receptors are functional. A) A mouse I7-green fluorescent protein (GFP) fusion protein expression cassette was introduced into cultured olfactory sensory neurons by recombinant lentiviral vector. I7-GFP expression recognized by GFP immunostaining (green) was detected in the olfactory sensory neurons identified by β-tubulin III expression (red). I7-GFP is distributed in the olfactory sensory neuron cell body, axon- and dendrite-like processes. B) Time course of the ratio of fura-2 fluorescence intensity at 340 nm and 380 nm (R340/380) recorded from olfactory sensory neurons expressing GFP alone (Bi) or I7-GFP (Bii and Biii) in the presence of 100 μM octanal (Bi and Biii) or 100 μM propionic acid (Bii). Arrows indicate timing of application of octanal or propionic acid. Each trace represents an average of R340/380 time courses recorded simultaneously from five to seven cells in a representative experiment. Bar = 20 μm.