Figure 3
Sfrp1, Sfrp1 CRD and Sfrp1 NTR mRNAs are translated at comparable levels when overexpressed in vivo. Western blot (WB) analysis of lysates from embryos injected with equimolecular amounts of Sfrp1-3xHA, Sfrp1 CRD -3xHA or Sfrp1NTR-3xHA mRNAs together with GFP mRNA as a tracer. Embryos were collected at St26 and their lysates were precipitated with a polyclonal anti-HA and blotted with monoclonal anti-HA. To account for possible variations in the amount of injected mRNA, the expression levels of Sfrp peptides were normalized against those of the co-injected EGFP protein. Note that the normalised density values of the three peptides (NDV) are very similar. Note also that SfrpCRD runs as a doublet that may represent monomeric and dimeric forms (arrows) or post-translational modifications. IP, immunoprecipitation.