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Figure 2 | Neural Development

Figure 2

From: The Netrin-related domain of Sfrp1 interacts with Wnt ligands and antagonizes their activity in the anterior neural plate

Figure 2

Sfrp1 NTR , but not Sfrp1 CRD , mimics the phenotype induced by the over-expression of full-length Sfrp1 . (a-p) All the panels are dorsal views of embryos at stage 19–20 (optic vesicle stage) injected with GFP mRNA alone (control) (a,e,i,m) or together with olSfrp1 (b,f,j,n), Sfrp1 CRD (c,g,k,o) or Sfrp1 NTR (d,h,l,p) mRNA. Embryos in (i-l) have been processed for double in situ hybridization with rx3 (red) and foxA2 (blue) probes. All other embryos were hybridized for one probe as indicated. Note how anterior markers are dramatically expanded in both the Sfrp1 and Sfrp1 NTR injected embryos (arrow in e-h), while over-expression of Sfrp1 CRD leads to a reduction of forebrain structures. Sfrp1 injected embryos also display axial duplications (j) and posterior truncations (b,j, arrow in n). See Table 1 for details. Scale bar: 0.1 mm.

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