Figure 7

Met is required for aspects of CaP identity. (a) A met MO-injected mn2Et (green) transgenic embryo labeled with znp1 antibody (red) showing that CaP has both a peripheral axon (arrow) and a central axon (asterisk). (a') The same cell showing only GFP. (a") The same cell showing only znp1 labeling. (b,c) Islet1/2 antibody (Isl; red) and zn1 plus znp1 antibodies (green) labeling showing that CaPs in met MO-injected embryos (c) co-express these markers as they do in controls (b). For each condition we examined eight spinal hemisegments per embryo. n = 56 met MO-injected embryos and 32 control embryos. (d) chat is expressed in somata in the normal location of PMNs; gray lines show segment boundaries. (e,f) Expression of chat (green) and the CaP and VaP-specific marker islet2 (red) shows that in met MO-injected embryos (f) CaPs express chat, as they do in controls (e). For each condition we examined eight hemisegments per embryo. n = 24 met MO-injected embryos and 15 control embryos. (g) Co-expression of chat (red) and met (green) in PMNs. Scale bars, 10 μm.