Figure 8

Dendritic growth, branching and targeting are regulated independently. (a,b) Three-dimensional reconstructions from confocal image stacks of RP2 neurons at 25–31 hours AEL and visualised with UAS-mCD8::GFP generated with AMIRA software. (a) Control. (b) Misexpression of GSd421 causes aberrant dendritic targeting to the posterior. Brackets in (a) indicate the dendritic tree. (a',b') Dendrograms derived from the reconstructions with branch points highlighted in magenta and the cell body and axon offset from the dendritic tree by green. (c) Quantification of the dendritic architectures for controls (green, N = 4) and GSd421 expressing RP2 neurons (magenta, N = 4). The significance of pair-wise comparisons using Student's t-test is indicated. Error bars indicate the standard error. Anterior is left and the ventral midline is down. Scale bar: 10 μm.