Figure 7

Displacement of dendritic branches from medial anterior to posterior lateral regions. RP2 neurons at 25–31 hours AEL and visualised with UAS-mCD8::GFP in the context of FascicilinII positive axon bundles (magenta) demarcating the medial and lateral neuropile (maximal Z-projections of confocal image stacks). (a) Control. (b) Misexpression of UAS-robo-Y-F (activated robo) leads to a lack of dendritic innervation of the medial neuropile (normally located anterior to the axon (arrowhead in (a)) and a concomitant expansion of dendrites in the lateral neuropile posterior to the axon (arrowhead in (b))). Dendritic extent anterior or posterior to the axon is indicated by brackets. (c) Quantification of anterior, posterior and total (combined) maximal dendritic extent for controls (green, N = 10) and UAS-robo-Y-F expression RP2 neurons (magenta, N = 8). The significance of pair-wise comparisons using Student's t-test is indicated. Anterior is left and the ventral midline is down. Scale bar: 20 μm.