Figure 3
From: Identification of Phox2b-regulated genes by expression profiling of cranial motoneuron precursors
Sox13 is down-regulated by Phox2b-expressing cells in the hindbrain. (a-f) In situ hybridizations on transverse sections of ventral r2 from wild-type (a-d) or Mash1-/- (e, f) E9.5 (a, b) or E10.5 (c-f) mouse embryos with DIG-labeled riboprobes recognizing Sox13 (a, c, e) or Phox2b (b, d, f). At E9.5, Sox13 expression is excluded from the ventral progenitor domain expressing Phox2b, but invades the progenitor domain when Phox2b expression has disappeared at E10.5 in wild-type (arrowhead), but not in Mash1-/- embryos. The Phox2b-expressing region as visualized in (b, d, f) is delimited by white bars. (g-j) In situ hybridizations on transverse sections of ventral r4 of HH18 (g, h) or HH24 (i, j) chicken embryos with DIG-labeled riboprobes recognizing Sox13 (g, i) or Phox2b (h, j). Asterisks mark a lateral Phox2b+ progenitor domain, which does not express Sox13.