Figure 2
HOP does not regulate the proliferation of SGL astrocytic stem cells. (a-c) After a 2 h pulse of BrdU, mice were processed for HOP/BrdU immunocytochemistry. Some HOP immunoreactive cells incorporate BrdU. Scale bar: 10 ÎĽm. (d, e) After a 2 h pulse of BrdU, mice were processed for GFAP/BrdU ICC and the double-labeled SGL cells were counted under a confocal microscope (n = 5). The data are presented as the number of GFAP/BrdU cells per mm of SGL. The number of GFAP/BrdU cells is unchanged in the HOP knock out mice compared to the wild type (d) even when growth factors (EGF + bFGF, 20 ÎĽg/ml) were infused into the lateral ventricle to stimulate the proliferation of DG progenitors (n = 5) (e). (f-h) HOP (g) or control (f) siRNA coupled to the cell permeant peptide Penetratin (20 ÎĽM) were infused for three days above the DG to locally silence HOP expression. A strong reduction of HOP expression is seen after HOP siRNA infusion. (h) The number of GFAP/BrdU cells is not significantly different between the two conditions (n = 5). Scale bars: 50 ÎĽm.