Figure 3
From: UV laser mediated cell selective destruction by confocal microscopy
UV cell destruction of ventral wing epithelial cells does not affect dorsal cells. (a-d) GFP labeling prior to (a,b) and after (c,d) UV cell destruction in fly wing epithelium. Dorsal views are shown in (a,c). The wing epithelium presents two cell layers as shown on transversal sections in (b,d). Dorsal epithelium (de) is to the top, ventral epithelium (ve) to the bottom. In each panel, acquisition is made from dorsal to ventral. The same color coding as described in Figure 1 is used to quantify GFP labeling. Before (b) and after (d) irradiation, the ventrally located targeted cell is indicated by a red arrow (b) and a white asterisk (d), respectively. Note that the dorsal GFP-positive cells (white arrowheads in (d)) located just above the ventral targeted cell are still present upon UV irradiation, even though they display a reduced fluorescence signal. See also Additional file 6.