Figure 2
From: UV laser mediated cell selective destruction by confocal microscopy
Effects of UV irradiation versus bleaching. (a,b) Color coding of confocal images of repo::GFP expressing cells in strong bleaching (a) or mild UV irradiation (b) experiments. (a) The distal glial cell (d) has been targeted using the 488 nm ray. GFP completely disappears from the cell within 5 minutes after bleaching (asterisk) and subsequently resumes (arrowhead). Notice that both targeted (d) and non-targeted (p) cells divide and that they do it almost simultaneously. See also Additional file 3. (b) The distal cell (d) has been targeted for mild UV irradiation; 54 minutes to 1 hour afterwards, the d cell prunes its cytoplasmic processes and undergoes fragmentation (small arrowheads). Finally, between 2.25 and 3 hours after UV irradiation, the d cell loses contact with its neighbors, acquires a round shape and moves away (arrow). See also Additional file 4. Scale bars: 25 μm.