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Figure 1 | Neural Development

Figure 1

From: UV laser mediated cell selective destruction by confocal microscopy

Figure 1

High resolution images of confocal assisted UV laser selective destruction. (a,b) Confocal images of repo::GFP expressing cells prior to (a) and after (b) selective destruction. (b) The proximal glial cell (p) has been targeted for selective destruction; the distal cell (d) was not targeted. (c,d) actin-GFP labeling in the fly wing epithelium prior to (c) and just after (d) UV irradiation. The region of interest defined by the 'Point bleach' function is indicated by the black square in (c). (d) Notice that, upon irradiation, GFP labeling is specifically absent in the targeted nucleus. Images to the bottom of (c,d) correspond to Z optical sections taken along the axis indicated by the black arrowheads. Color coding is used to quantify GFP labeling: blue (0) corresponds to background, red (250) to high levels. (e-h) actin-GFP labeling in the fly wing epithelium prior to (e) and 10 (f), 22 (g) or 32 minutes (h) after UV irradiation. The two targeted cells (indicated by red arrows in (e)) are absent 10 minutes after UV-mediated destruction (indicated by white asterisks in (f)). The space previously occupied by the targeted cells is subsequently occupied by the neighboring cells (g,h). See also Additional file 5. Scale bars: (a,b) 25 μm; (c,d) 5 μm; (e-h) 10 μm.

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