Figure 4

Tagged endogenous proteins localize to distinct neuronal compartments. Homozygous fly lines containing GFP transposon insertions were imaged by confocal microscopy. Single confocal sections of third instar brains are shown. The images were all acquired at the depth of the mushroom body calyx; the calyx region is indicated in each image with an asterisk. A diagram of a section through a third instar brain is shown at the upper left. One brain lobe (boxed region) is shown in each panel. At the top right an example of a GFP transposon insertion that yields cytoplasmic fluorescence in the cell body, axons and dendrites is shown. The bel, Pdi, and Map205 genes all contain Wee-P [22] insertions, and the Jupiter line was previously described [23]. Scale bar at lower left is 50 μm.