Figure 3

Olfactory projection neurons have molecularly distinct compartments. Tagged markers were expressed in olfactory projection neurons using the GH146 Gal4 driver, which is specific to these neurons and a few other scattered groups of neurons in the brain [56]. Brains were dissected from third instar larvae and fixed. Signal in nod-YFP and NgCAM-YFP panels was amplified by staining with GFP antibodies. Confocal projections through the region occupied by projection neurons are shown. The arrow in the nod-YFP panel represents fluorescence from other neurons; the asterisk indicates the projection neuron axons terminate; arrowheads indicate the beginning of the axons, just distal to the dendrite branch point. Scale bar is 20 μm.