Neurotrophin/Trk receptor signaling mediates C/EBPα, -β and NeuroD recruitment to immediate-early gene promoters in neuronal cells and requires C/EBPs to induce immediate-early gene transcription

Table 1 Primer sequences used for RT-PCR, q PCR and ChIP

	Gene	Sense	Antisense
RT-PCR	Cebpa	aaggccaagaagtcggtgga	cagttcacggctcagctgtt
	Cebpb	gcgcgagcgcaacaacatc	tgcttgaacaagttccgcag
	Neurod	tcaaccctcggactttcttg	gcagtcagttagggggcttt
	Ubiquitin	tggctattaattattcggtctgcat	gcaagtggctagagtgcagagtaa
qPCR	Egr1	gttatcccagccaaacgactc	ggttcaggccacaaagtgtt
	Fos	cccatccttacggactccc	gagatagctgctctactttgcc
	Egr2	ggaccacctctactctccg	tgggatcataggaatgagacctg
	Cebpa	gtcactggtcaactccagcac	caagaacagcaacgagtaccg
	Cebpb	ggagacgcagcacaaggt	agctgcttgaacaagttccg
	Neurod	atgaccaaatcatacagcgagag	tctgcctcgtgttcctcgt
	MAP2A/B	aaagttgcctccagttccattt	tctttgattccgtgggcattt
	gapdh	aactttggcattgtggaagg	acacattgggggtaggaaca
ChIP	Promoter Egr1	tgcccaccactcttggatgg	ttcaagggtctggaacagca
	CD Egr1	gttatcccagccaaacgactc	ggttcaggccacaaagtgtt
	Promoter Fos	gctcgccttctctgcctttc	gcgctctgtcgtcaactcta
	Promoter Fos*	tccctccctcctttacacag	cccgtcttggcatacatctt
	3' UTR Fos	ggctacactgtgagatccta	acaactgccagggccattag
	Promoter Egr2	gccctgttcctcagtccata	gccaggagttgctggtgtag
	CD Egr2	ggaccacctctactctccg	tgggatcataggaatgagacctg

For the ChIP experiments the same primers were used for both PCR and qPCR except where noted. *Additional primers used for Fos qPCR after ChIP. CD, coding region; UTR, untranslated region.

ISSN: 1749-8104