NeuroD expression is normal in Cebpa/b and trkB/C deficient brains. (a) NeuroD protein expression in Cebpa/b deficient brains or trkB/C deficient brains compared to controls. Blots were re-probed with anti-Sp3 antibodies to control for protein loading. P > 0.5 in both cases. (b) Densitometric analysis of NeuroD protein levels in cortical lysates of controls, Cebpa/b and trkB/C mutant mice. (c) MAP2 mRNA levels were not affected in Cebpa/b deficient brains. qRT-PCR was performed on total mRNA from newborn forebrains of control (Cebpalx/lx;Cebpb+/-) and mutant (Cebpalx/lx;Cebpb+/-;NesCretg/+) mice using primers specific for MAP2A/2B isoforms. After normalization to ubiquitin, mRNA levels were expressed relative to those for the control animals, which were given the arbitrary value of 1. Presented is a representative result of two independent experiments. Standard error is for three animals of each genotype.