BDNF dependent expression of Fos, Egr1 and Egr2 is affected in mice with compound Cebpa/b null mutant alleles. (a-c) qRT-PCR analysis was performed on mRNA obtained from E13.5 primary cortical neurons of wild-type and different genotypes for Cebpa/b null mutants as indicated. After 3DIV, the neurons were either left unstimulated or stimulated with 5 ng/ml of BDNF for 1 hour. The mRNA levels are relative, after normalization to gapdh, considering the levels for the stimulated wild-type cortical neurons as 100. Error bars represent the standard error obtained from three animals for each genotype. P < 0.0001 for all induced samples of mutants compared to wild type. (d) BDNF/TrkB dependent signaling in primary cortical neurons with mutant Cebpa/b. E13.5 cortical neurons derived from wild-type or different genotypes for Cebpa/b null mutants after 3DIV were either left unstimulated or stimulated with 5 ng/ml of BDNF for 5 minutes. Cell lysates were subjected to SDS-PAGE followed by immunoblotting with an antibody against the phosphorylated forms of ERK (p42/p44). The blots were re-probed with an anti-phospho-AKT antibody, and a second time with an antibody against α-tubulin to control for the amount of protein loaded.