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Figure 10 | Neural Development

Figure 10

From: Neurotrophin/Trk receptor signaling mediates C/EBPα, -β and NeuroD recruitment to immediate-early gene promoters in neuronal cells and requires C/EBPs to induce immediate-early gene transcription

Figure 10

Neocortical development in mice with compound Cebpa/b null alleles. Coronal sections of adult (12 weeks old) cortices of control (Cebpalx/lx;cebp+/-) and mutant (Cebpalx/lx;Cebpb+/-;NesCretg/+) mice were stained with (a,b) Cresyl violet, or immunostained for (c,d) NeuN. (e,h) Representative western blots for NeuN and GFAP protein levels in adult cortical lysates from control and mutant mice. To control for protein loading, blots were re-probed with an anti-Erk1 antibody. (f,g,i) Densitometric analysis of NeuN, Tju1, and GFAP protein levels in adult cortical lysates of control and mutant mice. (j,k) Immunostaining for MAP2 in control and mutant mice. (l) Representative western blots of MAP2 protein levels in adult cortices of mutant (Cebpalx/lx;Cebpb+/-;NesCretg/+) and control (Cebpalx/lx;Cebpb+/-) mice. To control for protein loading, blots were re-probed with anti-PLCγ antibodies. (m) Densitometric analysis of MAP2 protein levels reveals significant decrease in mutant compared to control mice. *P = 0.019. (n,o) Mean thickness of primary and secondary dendrites of pyramidal neurons located in the primary somatosensory cortex (visualized with the Golgi method). II to VI indicate the different cortical layers. Scale bars (a-d,j,k): 100 μm.

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