Figure 7

Acetyl-histone H3 profiling of Brn3a target gene loci. Histone H3 acetylation was assayed using ChIP and tiled PCR primer pairs from -10 kb to 15 kb of the Msc, Tcfap2b, NeuroD4 and Gata3 gene loci. Positive controls for these assays included primer pairs located in the promoter regions of the Mapt (tau), Gapdh, and Eno2 loci, which are highly expressed in the DRG and TG and are unchanged in Brn3a null mice. The silent alb1 locus was used as a negative control and to set the baseline of one-fold enrichment (yellow line). H3 acetylation of these loci in chromatin samples from the E13.5 DRG and TG were compared by t-tests using fold enrichment values for primer pairs flanking the transcription start site of each locus (points shown in red). H3 acetylation was not significantly different at the msc locus (p = 0.25), while Tcfap2b (p = 0.0003) and NeuroD4 (p = 0.0005) showed significantly greater acetylation in the TG, and Gata3 (p = 0.09) showed a trend toward greater acetylation in the TG. UTR, untranslated region.