Figure 6

Trigeminal-specific targets of Brn3a regulation. (a) TG data from a previously published data set [20]. (b) DRG data from the present study. Brn3a wild-type (WT), heterozygote (Het) and knockout (KO) E13.5 DRG were analyzed for global gene expression using U74Av2+U74v2B arrays to allow direct comparison with the prior analysis of the embryonic TG (Additional file 3). Msc, shown for comparison, exhibits markedly increased expression in the DRG and TG of Brn3a knockout mice, while Gata3, Tcfab2b, Calb2, Rab3b and Neurod4 are increased only in the DRG. Changes in Msc, Gata3, Tcfap2b, Calb2 and NeuroD4 have been previously verified in the TG of Brn3a knockout mice by in situ hybridization or immunofluorescence [20]. Rab3b is newly identified as a Brn3a target in the TG due to progress in the annotation of the mouse genome. Gene abbreviations in parentheses indicate expression below the statistically significant threshold of detection (absent call) in all three genotypes.