Figure 7
From: Cerebellum morphogenesis: the foliation pattern is orchestrated by multi-cellular anchoring centers
Bg fibers converge on the anchoring centers. (a-e) Anti-RC2 (red) and anti-BrdU (green) immunostaining of midsagittal section of the E16.5 Cb. (b) Bg fibers (arrows) located around the anchoring centers (asterisk) are oriented perpendicular to the outer surface of the Cb. (f-j) Anti-RC2 (red) and anti-BrdU (green) immunostaining of a midsagittal section of the E17.5 Cb reveals that, at the onset of fissure formation, Bg fibers (arrows) remain parallel to each other. (k-s) Anti-BLBP (red) and anti-BrdU (green) immunostaining of midsagittal sections of the E18.5 Cb, at the base of fissure (l-o) and at the crown of the folium (p-s), reveal a unique orientation of Bg fibers (arrows). (l) Bg fibers (arrows) in the anchoring centers fan out from a single point (asterisk). (p) Conversely, the Bg fibers (arrows) at the crown of the folia remain aligned perpendicular to the pial surface of the Cb. Cb, cerebellum; EGL, external granular layer. Scale bars: (a, f, k) 100 μm; (b-e, g-j, p-s) 15 μm.