Figure 8
From: Tissue interactions in the developing chick diencephalon
CYP1B1 is expressed in the dorsal diencephalon and is able to suppress ZLI formation. (A) Stage HH15 chick head, in situ hybridisation for expression of CYP1B1. Note expression in the dorsoanterior part of the eye, anterior to the MHB and in the epithalamus (red arrowhead). (B-D) Stage HH16 embryos were electroporated with CYP1B1-IRES-GFP, cultured to stage HH21/22 and analysed by in situ hybridisation for expression of Shh followed by immunochemical detection of green flourescent protein (GFP; lateral views of hemisected brains, anterior to the left, dorsal to the top). (B) Non-electroporated control side (co). (C, D) Electroporated side (ep); (D) an overlay with the anti-GFP fluorescent signal in green. Note reduced Shh expression in the ZLI even in areas where only few cells are transfected (red arrowhead), suggesting a non-autonomous effect.