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Figure 2 | Neural Development

Figure 2

From: Tissue interactions in the developing chick diencephalon

Figure 2

The ZLI is specified before stage HH10. (A) Explanted brain regions are schematically shown in a stage HH14 chick brain stained by in situ hybridisation for the expression of Lfng (anterior to the left, dorsal to the top). (B) Pro-Pth, pro-ZLI and pro-Th explants from stage HH14 brains were analysed by in situ hybridisation for the expression of Shh (left) and Lfng (right) at the time of dissection (pre-cult.) or after culture for 24 hours (post-cult.). Note induction of Shh in cultured pro-ZLI explants and absence of Lfng expression in pro-ZLI explants at the time of dissection and after culture. (C) Pro-Pth, pro-ZLI and pro-Th explants from stage HH10 brains. Note induction of Shh and absence of Lfng expression in cultured pro-ZLI explants. Lfng is expressed in pro-ZLI explants at the time of dissection.

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