Table 3 Rescue of olrn-1, overexpression of olrn-1, and effect of mutations
| Â | Â | Â | Percentage of animals (%) | Â | ||
|---|---|---|---|---|---|---|
Test strain | Test clone(concentration) | Line | 2AWCON | 1AWCON/1AWCOFF | 2AWCOFF | n |
olrn-1 | None | Â | 0 | 1 | 99 | 1,189 |
olrn-1 | odr-3::olrn-1b | 1 | 18 | 81 | 1 | 582 |
|  | (2.5 ng/μl) | 2 | 1 | 77 | 22 | 218 |
olrn-1 | odr-3::olrn-1b | 1 | 48 | 51 | 1 | 639 |
|  | (5 ng/μl) | 2 | 55 | 43 | 2 | 806 |
olrn-1 | odr-3::olrn-1b | 1 | 47 | 44 | 9 | 245 |
|  | (15 ng/μl) | 2 | 52 | 41 | 8 | 293 |
WT | odr-3::olrn-1b | 1 | 63 | 37 | 0 | 1528 |
|  | (15 ng/μl) | 2 | 70 | 29 | 1 | 1282 |
WT | odr-3::olrn-1b | 1 | 68 | 32 | 0 | 660 |
|  | (25 ng/μl) | 2 | 70 | 30 | 0 | 781 |
olrn-1 | odr-3::olrn-1b::Ch | 1 | 81 | 16 | 3 | 73 |
|  | (15 ng/μl) | 2 | 83 | 14 | 3 | 70 |
| Â | Â | 3 | 76 | 22 | 2 | 88 |
olrn-1 | odr-3::olrn-1b(ΔrawR1)::Ch | 1 | 0 | 4 | 96 | 94 |
|  | (15 ng/μl) | 2 | 0 | 1 | 99 | 91 |
| Â | Â | 3 | 0 | 8 | 92 | 39 |
olrn-1 | odr-3::olrn-1b(ΔTM1,2)::Ch | 1 | 49 | 51 | 0 | 94 |
|  | (15 ng/μl) | 2 | 53 | 46 | 1 | 96 |
olrn-1 | odr-3::olrn-1b(ΔrawR2)::Ch | 1 | 61 | 36 | 2 | 88 |
|  | (15 ng/μl) | 2 | 81 | 19 | 0 | 83 |
| Â | Â | 3 | 62 | 27 | 11 | 45 |
olrn-1 | odr-3::olrn-1b(G466E)::Ch | 1 | 6 | 86 | 7 | 81 |
|  | (15 ng/μl) | 2 | 1 | 96 | 3 | 91 |
| Â | Â | 3 | 5 | 91 | 5 | 43 |
olrn-1 | odr-3::olrn-1b(ΔRRRR)::Ch | 1 | 4 | 95 | 1 | 100 |
|  | (15 ng/μl) | 2 | 20 | 78 | 2 | 110 |
| Â | Â | 3 | 2 | 74 | 23 | 47 |
| Â | Â | 4 | 7 | 88 | 5 | 152 |
olrn-1 | odr-3::olrn-1(ΔCterm)::Ch | 1 | 0 | 2 | 98 | 51 |
|  | (15 ng/μl) | 2 | 0 | 0 | 100 | 88 |
| Â | Â | 3 | 0 | 0 | 100 | 47 |