Figure 3

Structure-function analysis of odr-3::olrn-1::Cherry. (a) Mutants generated in odr-3::olrn-1b::Cherry affected Raw repeats (ΔrawR1, ΔrawR2), potential transmembrane domains (ΔTM1,2), the four adjacent arginines (ΔRRRR), and a carboxy-terminal region (ΔCterm). The ky626 mutation (G466E) was also introduced. (b) Phenotypes of ky626 animals expressing odr-3:olrn-1b::Cherry transgenes. olrn-1(ky626) control is at left. All transgenes except ΔCterm showed significant rescue compared to nontransgenic sibling controls; all transgenes except rawR2 were significantly less active than intact odr-3::olrn-1b::Cherry (P < 0.008 by Fisher exact test or Chi square test as appropriate; 0.008 was used as the significance level based on the conservative Bonferroni correction for six comparisons; n > 100 rescued animals per clone, from at least two independent transgenic lines that showed similar degrees of rescue (Table 3)).