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Figure 2 | Neural Development

Figure 2

From: Left-right olfactory asymmetry results from antagonistic functions of voltage-activated calcium channels and the Raw repeat protein OLRN-1 in C. elegans

Figure 2

olrn-1 encodes a protein with Raw repeats and potential transmembrane domains. (a) Genomic structure of olrn-1, showing alternative first exons for a and b isoforms, whose 5' ends are separated by 3.8 kb. (b) Translation of olrn-1, showing alternative first exons for olrn-1a and olrn-1b isoforms. Two short repeats shared with Drosophila Raw are highlighted in green with conserved residues in bold type; potential transmembrane domains are boxed. The location of the splice acceptor site (fourth exon) mutated in ut305 and the residue mutated in olrn-1(ky626) (G 473a/466b E) are marked. Arrow marks the carboxy-terminal insertion site of Cherry in odr-3::olrn-1b::Ch (f). (c) Expression of olrn-1a::Cherry promoter fusion. Arrowhead indicates Cherry expression in AWC neuron expressing str-2::GFP. (d) Expression of olrn-1b::Cherry promoter fusion in non-neuronal cells. Arrows, hypodermal cells. The pharynx is a prominent site of expression. Arrowhead, no Cherry expression in AWC neuron expressing str-2::GFP. (e) Expression of amino-terminally tagged odr-3::Cherry::olrn-1b in an L4 olrn-1(ky626) animal. odr-3::Cherry::olrn-1b is excluded from the nucleus and is punctate in the axon and dendrite. (f) Expression of carboxy-terminally tagged odr-3::olrn-1b::Cherry in an L4 ky626 animal. odr-3::olrn-1b::Cherry is excluded from the nucleus, and is punctate in the axon and dendrite. Arrowheads, AWC cell bodies. Scale bars, 20 μm. Images are stacked confocal images.

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