Figure 5

Targeted expression of TeTxLC, Shi^ts1, Kir_2.1 and Glued-DN affects the architecture of the CSDn. Adult brains were stained with anti-GFP (green) and mAbnc82 (red). (a, g) Wild-type (WT) CSDn showing the cell body with dendrites in the ipsilateral lobe (arrows in (a)) and terminal arbors in the contralateral lobe (g). (b, h) The neuron expressing inactive tetanus toxin (UAS-IMPTNT-V/+; RN2-Flp, Tub-FRT-CD2-FRT-Gal4, UAS-CD8GFP/+) have dendrites (arrow in (b)) and terminal arbors (h) comparable to WT. (c, i) Ectopic expression of active form of tetanus toxin (UAS-TNT-G/+; RN2-Flp, Tub-FRT-CD2-FRT-Gal4, UAS-CD8GFP/+) results in dramatic increase in ipsilateral dendrites (arrows in (c)) and a marked decrease in contralateral terminals (i). (d, j) UAS-shi^ts1/+; RN2-Flp, Tub-FRT-CD2-FRT-Gal4, UAS-CD8GFP/UAS-shi^ts1 showing greater profusion of dendrites in the ipsilateral lobe (arrows in (d)) and reduced terminal arbors (j). (e, k) RN2-Flp, TubFRT-CD2-FRT-Gal4, UAS-CD8GFP/UAS-EGFPKir_2.1 showing greatly increased dendritic branches within the ipsilateral lobe (arrows in (e)) and modest decrease in contralateral terminals (k). (f, l) UAS-Glued-DN/+; RN2-Flp, Tub-FRT-CD2-FRT-Gal4, UAS-CD8GFP/+ showing increased ipsilateral dendrites (arrows in (f)) and a reduction in contralateral terminals (l). Scale bar = 30 μm. (m, n) Number of branch points from the ipsilateral (m) (N = 7) and contralateral (n) (N = 5) arbors were quantified from all the genotypes. Bars represent the mean and standard error of mean of number of branch points. P-values were calculated using the unpaired Student's t-test *P < 0.05, **P < 0.01, ***P < 0.0001.