Figure 3

Eye phenotypes induced by intercellular injection of anti-Pax6 monoclonal antibody. (a-d) Intercellular distribution of FITC-labeled anti-mouse antibody injected at the blastula stage. Embryos were analyzed in toto (a,c) or using confocal microscopy (b,d) either 1 hour after injection (a,b) or at the shield stage (c,d). Note that the staining is concentrated in the intercellular space and is virtually absent from the cell interior. (e-m) Prototypical eye phenotypes (quantifications in Figure 4): Embryo 1 (e-g) has a severely reduced right eye (lateral view (e)) compared with left eye (lateral view (f)) and no right retina or lens (section in (g)). Embryo 2 (h-j) has a strongly reduced left eye (frontal view in (h), lateral view in (i)). The horizontal section (j) confirms the phenotypes and indicates that the left lens is also reduced (arrow). Embryo 3 has two reduced eyes with disorganized morphologies (lateral views in (k,l)) and a reduced and misplaced left lens (red arrow in (l,m)). In all horizontal sections, rostral is to the left.