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Figure 6 | Neural Development

Figure 6

From: Distinct roles of Hoxa2 and Krox20in the development of rhythmic neural networks controlling inspiratory depth, respiratory frequency, and jaw opening

Figure 6

Hyperplasia of the r1-derived dorsal pontine tegmentum in Hoxa2-/- mutants. Para-sagittal sections of the brainstem of (a, b, d, e, g) Hoxa2EGFP(lox-neo-lox)/+;r2::Cre (labeled Hoxa2EGFP/+;r2::Cre) or (c, f, h) Hoxa2EGFP(lox-neo-lox)/-;r2::Cre (labeled Hoxa2EGFP/-;r2::Cre) E16 mice at different latero-medial levels, from more lateral (KF level) (a-c), to more medial (LC level) (g, h) through an intermediate level (trigemino-facial level) (d, e). (a, d) Violet cresyl stainings from which the delimitations of the different brainstem nuclei appearing as lines in other panels were drawn. (b, c, e, f) Immunodetection of EGFP, showing r2-derived cells. Note the reduction of the r2-derived domain and the ventral expansion of r1-derived nuclei such as the estimated KF (arrowheads in (c)), PB and RtTg (arrowheads in (f)) in Hoxa2EGFP(lox-neo-lox)/-;r2::Cre mice. (g, h) Immunodetection of EGFP at the LC level showing reduction of the r2-derived territory in Hoxa2EGFP(lox-neo-lox)/-;r2::Cre; insets show immunodetection of PHOX2A, a marker for the LC noradrenergic neurons – note the ventral expansion in Hoxa2EGFP(lox-neo-lox)/-;r2::Cre mice (arrows in (h)). 5Mo, trigeminal motor nucleus; 7Mo, facial motor nucleus; Cb, cerebellum; KF, estimated position of the Kölliker-Fuse nucleus; LC, locus coeruleus; MVe, medial vestibular nucleus; PB, estimated position of the parabrachial nucleus; Pn, pontine nuclei; Pr5, principal sensory trigeminal nucleus; RtTg, reticulo-tegmental nucleus of the pons; Sp5, spinal trigeminal tract; Su5, supratrigeminal nucleus; vsc, ventral spinocerebellar tract.

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