Figure 5

Protein levels of WAVE/SCAR complex subunits in different mutant contexts. (a) Immunoprecipitation experiments in Drosophila S2 cytoplasmic cell extracts using the anti-HSPC300 antibody. From left to right: anti-HSPC300 immunoprecipitation, IgG immunoprecipitation, input (cytoplasmic extract). Proteins are indicated to the right, corresponding molecular weights to the left. (b) Quantitative analysis of CYFIP, Kette, SCAR, Abi and HSPC300 protein levels by western blot on third instar larval extracts of the following genotypes: wild type (WT); HSPC300 zygotic null; HSPC300 zygotic null and maternal hypomorph; CYFIP zygotic null. Proteins are indicated to the right, corresponding molecular weights to the left. β-tubulin represents a loading control.