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Figure 2 | Neural Development

Figure 2

From: HSPC300 and its role in neuronal connectivity

Figure 2

HSPC300 locus and mutants. (a) Exon/intron organization and resulting transcripts are shown for the genes PebIII, CG3163 and HSPC300. ATG and Stop (in grey) indicate open reading frames. EP indicates the P element insertion in the EP(2R)0506 line and UAS indicates the upstream activating sequence contained in the P element. (b) Molecular characterization of three excision lines. Line HSPC300Δ96.1(as well as non-depicted HSPC300Δ52.1, HSPC300Δ31.1alleles) represent an excision event affecting the entire HSPC300 gene and part of the PebIII gene. Line HSPC300Δ54.3displays intact adjacent genes (PebIII and CG3163) and P element. The red line represents integrated HSPC300-unrelated sequence, and the dotted line represents uncharacterized sequence. (c) RT-PCR on the wild-type (wt) and the HSPC300Δ54.3excision line. The left three lanes show products obtained from wild-type third instar larvae; the right three lanes show product obtained from HSPC300Δ54.3larvae. Note that HSPC300 transcripts are detected in the wild type (third lane from the left), but not in HSPC300Δ54.3larvae (sixth lane from the left). In contrast, both PebIII (fourth lane from the left) and CG3163 (fifth lane from the left) transcripts are detected in the wild type and in mutant larvae.

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