Figure 1

HSPC300 expression profile. (a) Quantitative analysis of HSPC300 mRNA levels by light cycler at indicated developmental stages. Quantification is relative to the housekeeping ribosomal protein 49 (rp49) mRNA. Bars indicate SEM. APF: After puparium formation. (b) Western blot analysis on protein extracts prepared from the Drosophila S2 cell line (S2 extract), using anti-HSPC300 antibody (HSPC300). The right lane represents equivalent amounts of protein extract from the S2 cell line, which transiently overexpresses HSPC300 upon transfection (S2 extract + HSPC300). β-tubulin (β-tub) represents a loading control. (c) HSPC300 immunolabeling of a whole mount embryo at stage 16; ventral view, anterior to the left. HSPC300 shows specific accumulation in CNS longitudinal connectives and commissures. The arrow and arrowheads show midline and motor neuron labeling, respectively. Scale bar: 50 μm.