Figure 5
DFsn genetically interacts with Highwire to restrain synaptic terminal growth. (a) Representative confocal images of muscle 4 synapses co-stained with DVGLUT (green) and FasII (red), in wild-type (WT), DFsnf06595, hiwND 51, hiwND 51; DFsnf06595, hiwND 9and hiwND 9; DFsnf06595third instar larvae. (b) Quantification of bouton number of muscle 4 synapses in WT, DFsnf06595, hiwND 51, hiwND 51; DFsnf06595, hiwND 9and hiwND 9; DFsnf06595third instar larvae (n = 27, 34, 27, 28, 22, and 39 cells, respectively). The number of boutons in the hiwND 51; DFsnf06595double mutant is increased compared to hiwND 51(p < 0.001). The number of boutons in the hiwND 9; DFsnf06595double mutant is not significantly different from hiwND 9(p > 0.5), demonstrating a lack of enhancement of the hiwND 9phenotype by DFsnf06595.