Figure 4

The RS box is sufficient to confer a specific retinal action on Drosophila OTD protein. (a) Results of lipofection of RPCs with GFP alone or with GFP+otd; counted cells were as indicated in the histogram (n), from 17 retinae for GFP, and 11 retinae for otd; PC, photoreceptor cells; HC, horizontal cells; BC, bipolar cells; AC, amacrine cells; GC, ganglion cells. (b) Results of lipofection of RPCs with GFP alone, with GFP+Xotx wild-type constructs or GFP+otd/Xotx chimeric constructs, as indicated; counted cells are indicated in the histogram (n), from 15 retinae for GFP, 18 retinae for Xotx2, 15 retinae for Xotx5b, 9 retinae for otd/Xotx2, and 11 retinae for otd/Xotx5b. (c) Results of lipofection of RPCs with GFP alone, or with GFP+otd/box2/5b chimeric constructs, as indicated. Counted cells are indicated in the histogram (n), from six retinae for GFP, nine retinae for otd/box2, and nine retinae for otd/box5b. The proportion of each cell type (a-c) is represented as average ± standard error of the mean. (d, e) Lipofected retinae with otd/box5b are enriched in photoreceptors (d), those with otd/box2 are enriched in bipolar cells (e); GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. (f) An example of GFP+otd/box5b-lipofected photoreceptor cell positive for IRBP probe after in situ hybridization (Fast Red detection). (g) A GFP+otd/box2-lipofected bipolar cell expressing Xvsx1 is shown following in situ hybridization (Fast Red detection).