Figure 2

Zac1^+m/- retinae develop an ectopic amacrine cell layer and supernumerary rod photoreceptors. (a-h) E18.5→8DIV retinal explants. DAPI-stained (a) wild-type and (b) Zac1^+m/- explants. Rhodopsin expression in (c) wild-type and (d) Zac1^+m/-+ ECL retinae. (e,e',f,f') Pax6 and (g,g',h,h') syntaxin expression in amacrine cells in wild-type (e,e',g,g') and Zac1^+m/-+ECL (f,f',h,h') retinae. Asterisks mark the ECL. The duplicated IPL is labeled by ipl' in (h'). Blue is DAPI counterstain. (i) Average of the absolute number of DAPI⁺ nuclei/layer in a standard counting field in wild-type (black bar; total DAPI⁺ nuclei counted in 30 fields; ONL: 23,700; INL: 9,870; GCL: 1,776), Zac1^+m/- without an ECL (grey bar; total DAPI⁺ nuclei counted in 9 fields; ONL: 6,615; INL: 2,826; GCL: 498 nuclei) and Zac1^+m/-+ECL (white bar; total DAPI⁺ nuclei counted in 27 fields; ONL: 26,175; INL: 11,968; GCL: 1,674). (j) Percentage of each retinal cell type based on total cell counts in wild-type (black bar; HC: 56 calbindin⁺/7,183 DAPI⁺; AC: 1,832 Pax6⁺/11,696 DAPI⁺; BP: 819 Chx10⁺/9,302 DAPI⁺; MG: 1,003 p27⁺/18,465 DAPI⁺ nuclei; 537 CRALBP⁺/9,169 DAPI⁺), Zac1^+m/- without an ECL (grey bar; HC: 64 calbindin⁺/12,960 DAPI⁺; AC: 1,558 Pax6⁺/10,304 DAPI⁺; BP: 1,077 Chx10⁺/10,171 DAPI⁺; MG: 430 p27⁺/9,966 DAPI⁺; 332 CRALBP⁺/6,773 DAPI⁺) and Zac1^+m/-+ECL retinae (white bar; HC: 11 calbindin⁺/1,924 DAPI⁺; AC: 2,068 Pax6⁺/11,302 DAPI⁺; BP: 646 Chx10⁺/9,157 DAPI⁺; MG: 395 p27⁺/9,921 DAPI⁺; 240 CRALBP⁺/3,319 DAPI⁺). AC, amacrine cell; BP, bipolar cell; HC, horizontal cell; MG, Müller glia.